Happy Independence Day, Happy Canada Day!

We’re situated in Rochester, NY – less than 100 miles from the US-Canadian border. In our part of the world, the Americans and Canadians just celebrated
their national birthdays. The city of Rochester has a rich history of imaging and optics. Eastman Kodak, Xerox and Bausch & Lomb were founded here. We’re proud to be part of the newest generation of optics and imaging companies
to come out of this region. Let us know how we can help you image better.

Regards,

The TEMwindows.com Team

Student Awards for Microscopy & Microanalysis 2010

Once again we are offering cash awards to students & post-docs who demonstrate use of our UltraSM® Pure Silicon, Silicon Oxide or
Silicon Nitride TEM windows. It’s not too late to apply. Click here for more details.

Platform Presentations

Christopher Striemer, PhD, Vice President of Membrane Development and Thomas Gaborski, PhD, President and COO will be each giving a platform presentation at M&M this year.

See Chris present details of our thin film membrane technology:

Tuesday August 3, 2010

A-01 Vendor Symposium: Creating the Tools for Science (Room B115)

1:45 PM 310 Advanced Imaging and Separation Tools Based on Ultra-thin Porous Silicon Membranes; CC Striemer, TR Gaborski,; SiMPore, Inc

See Tom present cell culture work using our UltraSM® Pure Silicon membranes:

Thursday August 5, 2010

B-06 Microfluidic Devices: Emerging Platforms for Live Cell Microscopy (Room B115)

9:45 AM 803 Highly Permeable Membranes for Live Cell Imaging of Co-Cultures; TR Gaborski; SiMPore Inc; BJ Nehilla; University of Rochester; CC Striemer; SiMPore Inc; JL McGrath; University of Rochester

Cell growth on nanoporous UltraSM® published in Biomaterials this month!

An article this month in Biomaterials illustrates how cells can be grown on nanoporous
UltraSM® membranes. From the abstract:

“The current study investigates the feasibility of pnc-Si as a cell culture substrate by measuring cell adhesion, morphology,
growth and viability on pnc-Si compared to conventional culture substrates. Results for immortalized fibroblasts and primary vascular endothelial cells are highly
similar on pnc-Si, polystyrene and glass. Significantly, pnc-Si dissolves in cell culture media over several days without cytotoxic effects and stability is tunable
by modifying the density of a superficial oxide. The results establish pnc-Si as a viable substrate for cell culture and a degradable biomaterial. Pnc-Si membranes
should find use in the study of molecular transport through cell monolayers, in studies of cell-cell communication, and as biodegradable scaffolds for three-dimensional
tissue constructs.”

Coming later this summer, SiMPore and TEMwindows.com will be offering UltraSM® products for cell culture imaging in both light and electron microscopy. Please
check our website for updates.

Annual Sample Pack Sale – Just $230

This month we are announcing our annual 50% off sale on our sample pack. The sample pack has a special price of $230 and includes the following items:

• 5 x Porous UltraSM Pure Silicon 15 nm TEM Windows (squares)
• 5 x Non-Porous UltraSM Pure Silicon 9 nm TEM Windows (squares)
• 5 x Silicon Oxide 20 nm TEM Windows (squares)
• 5 x Silicon Nitride 20 nm TEM Windows (single 500 micron square)

Click here for more details.

Custom TEM Grids From TEMwindows.com

Our team of engineers has the capabilities to develop and fabricate custom TEM grids and other suspended-membrane chips. We take pride in meeting your
custom specifications with tight tolerances. We can help you refine your design and develop a fabrication plan to meet your needs. Our custom capabilities
include fabrication of devices:

• 100 – 400 micron thick silicon frames
• Frame geometries from standard 2.9 mm TEM grids to 5 x 5 mm chips (and larger)
• Windows of 0.025 – 2.5 mm, depending on membrane types
• Membranes of pure silicon, silicon nitride or silicon oxide from 5 – 200 nm thick
• Other novel materials also available

The above image shows samples from a wide range of custom orders. Recent examples include:

• 2.9 mm TEM Grids with 30 nm silicon nitride membranes and 25 micron and 50 micron square windows (top row, first two grids) – produced for the Riken Research Institute in Japan.
• 5 x 5 mm chips with 125 nm sputtered silicon oxide membranes on 1 x 2.5 mm openings (top row, last grid on right) – produced for the Max-Planck Institute in Germany.

Learn more about our custom capabilities by downloading our PDF brochure. Most custom projects
are completed within 4-6 weeks.

Please contact us if you have any specialized grid requirements, and our engineers would be happy to provide feedback on potential solutions.

“Any sufficiently advanced technology is indistinguishable from magic.”

- Arthur C. Clarke

At TEMwindows.com and our parent company SiMPore, Inc., we are developing new products and applications for cell imaging.
Recent collaborations have shown the usefulness of our UltraSM® Pure Silicon grids for optical and SEM imaging of cells. Additionally, our
current product development efforts show promising application of our pure silicon, nanoporous, ultrathin membrane technology for cell growth and
imaging. An overview of these applications is described in further detail in this month’s e-newsletter.

We welcome your feedback and encourage you to contact us at sales@temwindows.com with any questions or suggestions you may have.

Regards,
The TEMwindows.com Team

Cell Imaging on UltraSM® Membranes

The UltraSM® silicon membrane provides a first-in-class combination of imaging and cell growth abilities for optical and electron microscopy.
This ultra-thin membrane permits imaging of cells by either light, fluorescent or electron
microscopic methods. The millions of nanopores within the membrane allow two cell types on either side of the membrane to communicate
with one another. The cells can be physically separated and imaged by optical means due to the excellent optical characteristics of the
UltraSM® membrane. Together, these properties make the UltraSM® silicon membrane an ideal substrate for growth and imaging of single or
multiple cell-type cultures. For information on cell growth and imaging on UltraSM®, click here.

UltraSM® membranes facilitate co-culture studies and cell imaging studies using confocal microscopy

Growth & Viability of Cells

Cell adhesion to, and observed growth rates on, UltraSM® silicon membranes are equivalent to conventional tissue culture
plastic or glass cover slips. The silicon composition of UltraSM® membranes is biocompatible, unlike carbon films on cytotoxic, metallic
grids. The native silicon oxide film on the membrane contributes a slight negative charge and a glass-like, cell-friendly surface for
attachment and growth.

The nanometer thickness of the UltraSM® silicon membrane is thin enough to allow light and
electrons to pass without significant diffraction. The millions of 10-50 nm diameter nanopores within this membrane create a highly
permeable film which allows cells grown on either side of the membrane to communicate with diffusible signaling molecules.

For multiple cell-type culture models, the UltraSM® membrane enables co-culture of multiple cell types in a setting
where the cells can communicate with one another by short-range signals and be maintained at physiologically meaningful distances across
the membrane. Combined with the ability to image and analyze both cells on either side of the membrane, the UltraSM® membrane is an ideal
substrate for many co-culture studies.

Optical Characteristics

Intracellular details can be resolved within cells grown on UltraSM® silicon membranes. Typical polymer membranes used for
cell culture are too thick to allow detailed resolution of intracellular structures and contribute significant background caused by membrane
auto-fluorescence. The silicon composition of UltraSM®
membranes eliminates concerns over auto-fluorescence, permitting fluorescent imaging at near-UV excitation and emission.

UltraSM® membranes provide ability to visualize intracellular detail

Electron Microscopy of Cells

Cells can be imaged on UltraSM® silicon membranes using electron microscopy methods as well. The added ability to
correlate light, fluorescent and electron microscopy images is possible. The silicon composition of UltraSM® membranes is sufficiently
conductive for SEM imaging without any carbon or gold coating and should allow current dissipation to prevent specimen charging under
more intense beam currents.

This example shows an image where neutrophils were fixed, dried and imaged by SEM (no staining or gold-sputtering was required). It is
also possible to culture cells on the grids prior to imaging. In this case, these neutrophils were pipetted onto the grids prior to
preparation.

“The greatest compliment that was ever paid me was when one asked me what I thought, and attended to my answer.”

- Henry David Thoreau

We feel fortunate to have an engaged group of customers who provide feedback and suggestions on our technology and products. Based on input
we have received, we are announcing several new products to better meet your needs. We are also lowering the prices on our products to make it even more affordable to switch from carbon grids to pure silicon, silicon nitride, or silicon oxide grids.

We look forward to continuing our dialogue with you!

Regards,
The TEMwindows.com Team

New Year = New Products & Pricing

New Products

Late in 2009, TEMwindows.com introduced 100 micron thick frame versions for several grid formats. We’ve expanded upon this, and the following products are now available in 100 micron thick frames:

• Pure silicon UltraSM® TEM Windows with 5 nm, 9 nm, or 15 nm thick membranes in both nine (9) square and two (2) slot formats.
• Silicon Nitride TEM Windows with 20 nm thick membranes in nine (9) square and two (2) slot formats.
• Silicon Oxide TEM Windows with 20 nm thick membranes in the two (2) slot format.

In addition, we have moved to an asymmetric format for our 100 micron products, to aid the researcher in identifying the same position when switching between light and electron microscopy.

See our website for details.

New Pricing

Prices on all TEMwindows.com films have been reduced by 10% to 30%. Our pure silicon UltraSM® TEM windows are now available for $21 to $29 per grid. And we are especially pleased to announce
that our Silicon Nitride grids can be purchased now for as low as $9 per grid. Silicon Oxide grid prices have also been lowered. It is our hope that these price reductions will make it even easier to experience the benefits of pure silicon, silicon nitride, and silicon oxide grids.

Our TEMwindows.com website has been updated with the new products and pricing. We’ve also reorganized it to make it easier to find the right grid for your particular application as well as answers to your technical questions.

See our website for details.

Human Neutrophil Activation on SEM Using UltraSM®

Pure silicon TEM windows are also excellent substrates for scanning electron microscopy (SEM). Due to the conductivity of the pure silicon film,
there is better charge dissipation than with oxide or nitride films eliminating the need for gold sputtering. Images required limited sample preparation.

Human neutrophils were placed on IL-8 coated pure silicon TEM window grids (15 nm thick) and allowed to activate. Cells were fixed with glutaraldehyde, then dried with increasing concentrations of ethanol.

Credit: Elena Lomakina, Rick Waugh and Brian McIntyre @ University of Rochester.

See our photoblog for more details.

New www.temwindowgrids.com Photoblog

We have been pleased with the response to our new photoblog, showcasing TEM images from TEMwindows.com customers. Click here to view the photoblog and we invite you to take a look at the images that are posted,
comment on them, and send us additional images to post. As a thank you, you’ll receive a $25 Amazon.com gift card for any images we receive that feature one of our TEMwindows.com grids. Please send your images to sales@temwindows.com.

4Q 2009 iPod Nano Winner

Boyan Penkov, University of Washington, Seattle, WA is the winner of our quarterly iPod drawing for being a new subscriber to the TEMwindows.com newsletter. Please forward our newsletter to colleagues who are interested in the latest materials research and TEM tools and encourage them to subscribe to the newsletter. We will plan to conduct another quarterly iPod drawing in April.