Pure silicon TEM windows are also excellent substrates for scanning electron microscopy (SEM). Due to the conductivity of the pure silicon film, there is better charge dissipation than with oxide or nitride films eliminating the need for gold sputtering. Both of these images required no sample prep beyond drying.
Human neutrophils were placed on IL-8 coated pure silicon TEM window grids (15 nm thick) and allowed to activate. Cells were fixed with glutaraldehyde, then dried with increasing concentrations of ethanol.
Credit: Elena Lomakina, Rick Waugh and Brian McIntyre @ University of Rochester. Click here to learn more about the neutrophil biophysics research at the University of Rochester.
Non-Porous UltraSM® 15 nm thick TEM Windows
Available with 2 slots or 9 squares
$230 for ten